DNA purification from plant materials plays a crucial role in various areas of plant research, including genomics, molecular biology, and genetic analysis essential for plant breeding and testing (e.g. GMO testing). The availability of high-quality DNA is essential for reliable results for a variety of applications including PCR, next generation sequencing, genotyping and chip technologies.
The sbeadex™ Maxi Plant Kit without Dangerous Goods is a comprehensive solution designed for extracting DNA from a wide range of plant species. This kit is optimised to efficiently extract and purify DNA from both fresh and lyophilized plant tissue material, including leaves, seeds, fruits, pulp, bark and roots.
The Maxi Plant Kit is best for large-scale purification with a recommended starting material of 10-30 mg for lyophilized tissue or 40-120 mg for fresh tissue. The kit is ideal for labs and researchers with higher throughput requirements and the need for larger scale DNA purification.
The DNA purification protocol, especially post optimization with our sbeadex Plant Trial Kit, is easy to automate, allowing for seamless scaling up of sample processing using popular robotic platforms.
The sbeadex Technology
This kit leverages the power of sbeadex magnetic bead technology. These superparamagnetic particles, coated with sbeadex surface chemistry, employ a novel two-bind / two wash mechanism that ensures effective removal of impurities from the sample matrix. The second wash buffer in the kit is ethanol-free, eliminating any traces of ethanol that could potentially inhibit downstream reactions. Additionally, the sbeadex technology eliminates the need for a drying step before elution, further enhancing DNA quality.
sbeadex plant kits have been successfully used with:
Plant species Leaves Seed
Apricot (Prunus armeniaca) X
Barley (Hordeum vulgare) X X
Beet, sugar (Beta vulgaris) X
Canola/oilseed (Brassica napus) X X
Chicory (Cichorium intybus) X
Corn (Zea mays) X X
Cotton (Gossypium) X X
Cucumber (Cucumis sativus) X X
Flax (Linum usitatissimum) X
Grape (Vitis vinifera) X X
Lettuce (Lactuca sativa) X
Muskmelon (Cucumis melo) X X
Onion (Allium cepa) X X
Parsley (Petroselinum crispum) X X
Peach (Prunus persica) X
Pepper (Capsicum annuum) X X
Potato (Solanum tuberosum) X
Rice, Asian (Oryza sativa) X X
Rubber (Hevea brasiliensis) X X
Soybean (Aphis glycines) X X
Sunflower (Helianthus annuus) X X
Tobacco leaves (Nicotiana tabacum) X X
Tomato (Solanum lycopersicum) X X
Wheat (Triticum L.) X X
Typical quantities of starting material are:
5-10 mg lyophilised tissue or 10-30 mg fresh tissue for the sbeadex Mini Plant DNA Purification Kit
10-30 mg lyophilised tissue or 40-120 mg fresh tissue for the sbeadex Maxi Plant DNA Purification Kit
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Key features
- Extract high-quality DNA from a wide variety of plant species and materials
- Contains no dangerous goods ensuring safety and regulatory compliance
- Suitable for large-scale DNA purification
- Suitable for all downstream applications
- Utilises sbeadex magnetic bead-based technology
Product Details
The sbeadex Maxi Plant Kit without Dangerous Goods contains all of the components required to perform large-scale DNA purification from plant tissue. As a Maxi Kit the volume of the kit components are doubled compared to the Mini Plant Kits.
The kit includes 2 wash buffers (concentrate), lysis buffer, elution buffer, binding buffer (concentrate), and sbeadex particle suspension. As a kit without dangerous goods the binding buffer and wash buffer concentrates contain no hazardous substances.
Choose between 96 or 960 preparations, or enquire for bulk options.
The kit is compatible with both fresh and lyophilised plant tissue material. The suggested starting material is 10-30 mg lyophilised tissue or 40-120 mg fresh tissue.
We highly suggest optimising your sbeabex DNA purification protocol first with our Mini Plant Trial Kit.
Resources
Application notes
oktopure and sbeadex plant nucleic acid extraction kit
sbeadex maxi plant kit and KingFisher 96 instrument
FAQs
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