sbeadex Lightning Cell DNA Kit enables superfast purification from human or animal cells using an innovative binding principle that allows simultaneous binding and washing of DNA. This patent-pending magnetic bead technology reduces the purification workflow from a 6-step to a 4-step process.
Despite its simplified and shortened protocol, sbeadex Lightning delivers highly pure DNA in yields comparable or higher to those of popular purification kits.
Advantages of the simplified protocol include:
Time savings for extraction, enabling faster acquisition of high-quality DNA
Reduced environmental impact due to lower hazardous liquid waste and plastic consumption
Lower associated costs for transport, storage, plastic consumables, and waste disposal
The sbeadex Lightning Cell DNA Kit is suitable for both manual and automated DNA isolation. Manual extraction from cells can be completed in approximately 11 minutes (refers to incubation times only and includes cell lysis). Automation protocols for KingFisher and oKtopure are available upon request. Contact us to discuss automation options.
For application data, please refer to the data sheet in the document section.
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Key features
- High DNA quality and yield: Attain highly purified DNA in high yields suitable for a wide range of downstream applications including NGS and qPCR
- Save time and increase sample throughput: Superfast and convenient protocols that is easy to scale up and automate on popular open access platforms including KingFisher, Dynamic Devices, Hamilton and our oKtopure.
- Cost savings: Benefit from a 4-step protocol that reduces overall lab costs (e.g. plastic consumables, waste disposal) and ensures reliable and reproducible results
- Sustainability and user safety: reduced plastic consumption, no chaotropic or ethanol bases wash buffers - reduced hazard and reduced packaging waste vs. traditional mag bead based kits.
Specifications
Input amount:
Input amount: 106 to 3 x 103 human or animal cells, fresh or frozen
Typical yield:
up to total yield of 20 µg gDNA depending on input amount and cell type
Protocol time:
11 minutes manual, 15 minutes KingFisher, approx. 25minutes for 2plates on oktopure platform
Elution volume:
50 - 200 µL
Compatible downstream applications:
NGS, qRT-PCR
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Product Details
The sbeadex Lightning Cell DNA Kit contains all reagents to isolate high-quality DNA from human and animal cells.
The Kit contains:
-Lysis Buffer CLA
-Protease K solution
-RNase A solution
-sbeadex particle suspension
-Binding Buffer LCL
-Elution Buffer AMP
Choose between 96 or 960 preparations, or enquire for bulk options.
Resources
Additional resources
EXTERNAL LINK
sbeadex Lightning Cell automation Analytik Jena app note
Documents
