Blood samples can present a myriad of challenges due to their inherent variability in quality. These variations can have a significant impact on downstream analyses, leading to inconsistent results and affecting the reliability of molecular testing protocols.
The sbeadex Blood DNA Purification Kit addresses these challenges by offering a robust and standardized method to overcome the inherent complexities of blood samples. The kit enables researchers to consistently obtain high-quality DNA from various blood sample types, including whole blood, buffy coats, and samples collected with different anticoagulants like EDTA, heparin, and citrate.
The kit's ease of automation and high-throughput capabilities allow it to seamlessly scale up sample processing using popular robotic platforms like the ThermoFisher™ KingFisher™ magnetic particle processor.
Additionally, the absence of PCR inhibitors in the final eluate, achieved through a water-based wash buffer, ensures reliable downstream analyses without the risk of interference from organic solvents or chaotropic salts.
The sbeadex Technology
This kit leverages the power of sbeadex magnetic bead technology. These superparamagnetic particles, coated with sbeadex surface chemistry, employ a novel two-bind / two wash mechanism that ensures effective removal of impurities from the sample matrix. The second wash buffer in the kit is ethanol-free, eliminating any traces of ethanol that could potentially inhibit downstream reactions. Additionally, the sbeadex technology eliminates the need for a drying step before elution, further enhancing DNA quality.
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Key features
- Ensures reliable and high-quality DNA preparations
- Compatible with various blood sample types, including whole blood, buffy coats, and common anticoagulants such as EDTA, Heparin, and Citrate
- Supports high-throughput processing and is compatible with common liquid handlers like KingFisher™
- Ideal for various downstream applications, including genetic disease screening, NGS, PCR/qPCR, library cloning, and Sanger sequencing
- Includes water-based wash buffers, minimizing solvent carryover and ensuring the absence of PCR inhibitors
Product Details
The sbeadex Blood DNA Purification Kit includes 3 wash buffers, 1 binding buffer, 1 lysis buffer, 1 elution buffer, protease K solution, and sbeadex particles suspension.
100 µL and 200 µL sbeadex blood protocol’s are available
Choose between 96 or 960 preparations, or enquire for bulk options
Resources
Application notes
automation sbeadex Blood Hamilton
sbeadex Blood Kit anticoagulant
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