Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where species of nucleic or ribonucleic acid are to be matched to complimentary sequences (Southern and Northern blotting, in situ hybridization, or DNA microarrays). SSC is used to help control the stringency of hybridization in these steps.
SSC-20x ph 7.0 is made of 3M sodium chloride and 0.3M sodium citrate in DEPC-treated water at pH 7.0. It is a concentrated stock solution filtered through 0.22 μm filter and autoclaved.
Applications:
Nucleic Acid Hybiridization.
Blot Transfer Procedures.
Nucleic Acid Preparation.
Contents & Storage:
20x SSC concentrated with 3M sodium chloride and 3M sodium citrate in DEPC-treated water at pH 7.0
Autoclave sterilization and filtered through 0.22μm filter
1 L/bottle
Store at Room Temperature for 1 year
Research use only.
Specifications
Storage
20x SSC concentrated with 3M sodium chloride and 3M sodium citrate in DEPC-treated water at pH 7.0
