rProtein A Protein G GraviTrap contains ten columns with 1 mL 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow resins.
For general-purpose purification of monoclonal and polyclonal antibodies.
Suitable when the affinity for protein A and protein G is not known.
GraviTrap prepacked gravity flow columns enable simple one-step purification of proteins in 30 min without the need for equipment.
High purity: > 95% in one step.
High protein recovery (typically 70% to 80%) due to high binding capacity of ~ 35 mg IgG/mL resin.
Fast and simple antibody purification
This column can be used for manual purification of polyclonal and monoclonal antibodies from cell culture supernatant and biological fluids. It is particularly suitable when the affinity for protein A and protein G is not known. The gravity flow column relies on gravity and is reusable up to five times, so no additional instruments are needed for this protocol.
Other Cytiva products for antibody affinity chromatography
Cytiva's solutions include a variety of resins, prepacked columns, and other formats for purifying antibodies and antibody fragments.
