RNase A Solution

RNase A (Ribonuclease A) is a single chain polypeptide with 4 disulfied bridges. It is an endoribonuclease that specifically cleaves single stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5’ribose of a nucleotide and the phosphate group attached to the 3′-ribose of an adjacent pyrimidine nucleotide. The resulting 2′, 3′-cyclic phosphate is hydrlized to the corresponding 3′-nucleoside phosphate.

Applications:

• Plasmid and genomic DNA preparation.
• Removal of RNA from recombinant protein preparations.
• Ribonuclease protection assays. Can also be used in conjunction with RNase T1.
• Mapping single-base mutations in DNA or RNA

Contents:

Research use only.

Documents

• MSDS (PDF)