RIPA Buffer

RadioImmunoPrecipitation Assay (RIPA) Buffer is used to lyse cells and tissues under non-denaturing conditions.

 

Directions for Use:

• Aliquotting of RIPA buffer is recommended if many small experiments are to be performed. For longer periods of time, the buffer should be stored at –20°C. If buffer will be continually used, it is recommended that the buffer be kept at 4°C for 1-2 weeks.
• Thaw RIPA buffer at room temperature, mixing end-over-end.
• Chill RIPA buffer on ice and add PMSF (final 1 mM) just prior to use.

 

Storage Conditions:

Supplied as a ready to use solution, 50mL. 100mM PMSF, 0.5mL.
Store at –20℃. For short-term storage (1-2 weeks), RIPA Buffer can be stored at 4°C.

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)

References

1. Pan, B., Zhan, X., & Li, J. (2022). MicroRNA-574 Impacts Granulosa Cell Estradiol Production via Targeting TIMP3 and ERK1/2 Signaling Pathway. Frontiers in endocrinology, 13, 852127. https://doi.org/10.3389/fendo.2022.852127

2. Pan, B., Liu, C., Zhan, X., & Li, J. (2021). Protegrin-1 Regulates Porcine Granulosa Cell Proliferation via the EGFR-ERK1/2/p38 Signaling Pathway in vitro. Frontiers in physiology, 12, 673777. https://doi.org/10.3389/fphys.2021.673777

Research use only.