RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.
Features
Accurate—Identify each transcript's strand of origin with >99% accuracy.
Sensitive—Detect low-abundance transcripts from as little as 100 pg of input RNA. It is recommended to use rRNA-depleted or poly(A)-purified RNA.
Integrated with Illumina sequencing—Incorporate Illumina indexes and adapters during PCR amplification.
Couldn't get easier—Go from start to finish in less than 4 hours.
Applications
RNA-seq for prokaryotic or mammalian samples on Illumina platforms
NGS library generation that retains strand information Analysis of coding and non-coding sequence information
