RHB-A®

RHB-A, supplemented with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor-2 (FGF-2), enables the maintenance and continual expansion of symmetrically-dividing NS cells in defined, serum-free adherent culture. In growth factor supplemented RHB-A, NS cells have been demonstrated to retain their neurogenic capacity for over 100 generations, with full maintenance of diploid karyotype. RHB-A in the presence of EGF and FGF-2 also supports the derivation of clonogenic NS cell lines. Culture of adherent NS cells in RHB-A with sequential growth factor withdrawal leads to differentiation into functional neurons. RHB-A supplemented with EGF and FGF-2 has recently been used for the propagation of glioblastoma stem cell lines.

Features

• RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells
• RHB-Basal medium is free of animal components and neuronal supplements; the medium can be customized by the addition of supplements

Documents

• Certificate of Analysis (PDF)
• User Manual (PDF)