Recombinant RNase Inhibitor ver.2.0 contains a mutation in a cysteine residue that is very sensitive to oxidation, making it highly stable in enzymatic reactions requiring low DTT concentrations. It inhibits RNase A activity through the formation of a 1:1 complex in a reversible reaction. It is not effective against RNase H activity.
Features
Efficient RNAse A inhibitor
Highly stable against oxidation
Ideal for applications requiring low DTT concentration, such as in vitro transcription, in vitro translation, or RT-PCR
Suitable for long-term storage of samples
Applications
In vitro transcription/translation (1 U/µl reaction)* In vitro transcription/translation with cell-free extract (20 U/µl reaction)* RT-PCR (0.5 U/µl reaction)* cDNA synthesis (0.5 U/µl reaction)* Polysome isolation (1 U/µl reaction)* *The parentheses show the recommended concentration for each appli
