Recombinant RNase Inhibitor forms a 1:1 complex with RNase A and inhibits RNase activity. This reaction is reversible, and ribonuclease activity can be recovered by dissociating the complex with urea or sulfhydryl reagent. This reaction irreversibly inactivates the inhibitor. Recombinant RNase Inhibitor can be added directly to reaction mixtures containing RNA. In addition, because it is a protein, it differs from other competitive inhibitors (nucleotides and inorganic phosphates) by being able to be easily removed from the reaction system using phenol extraction. Cat. # 2313B contains 5 of Cat. # 2313A. Please refer to Cat. # 2313A for complete product documentation and resources.
Unit definition
One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is determined as the ability to inhibit hydrolysis of cytidine 2,3'-cyclic monophosphate (cyclic CMP) by RNAse A
