Alternative names: Tryptase alpha/beta-1, TPSAB1, TPS1, TPS2, TPSB1
Molecular Weight: 28.2 kDa
Concentration: 0.5mg/ml
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Purity: > 90% by SDS-PAGE
Endotoxin level: < 1 EU per 1ug of protein (determined by LAL method)
Tag: His-Tag
Application: SDS-PAGE, Tryptase Enzyme Activity
Storage Condition: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
TPSAB1, also known as tryptase alpha/beta-1, is a serine protease with trypsin-like specificity. It is the key neutral protease present in mast cells and is discharged upon the coupled activation-degranulation response of this cell type. This protein is enzymatically active only as a heparin-stabilized tetramer, and is resistant to all known endogenous proteinase inhibitors. Also, it is implicated as a mediator in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
