Recombinant DNase I (RNase-free) is an endonuclease that catalyzes, to the same degree, the random degradation of both single- and double-stranded DNA and produces 5'-P terminal oligonucleotides. This enzyme does not exhibit protease activity. This enzyme is stable around its optimum neutral pH range and is suitable for RNA preparation at neutral pH. Cat. # 2270B contains 5 of Cat. # 2270A. Please refer to Cat. # 2270A for complete product documentation and resources.
Features
Template DNA digestion following in vitro transcription
Genomic DNA digestion prior to RT-PCR
Nick translation with DNA polymerase
Applications
Template DNA digestion following in vitro transcription Genomic DNA digestion prior to RT-PCR Nick translation with DNA polymerase Shotgun DNA library construction Footprinting analysis
Unit definition
One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 25°C (pH 5.0) using calf thymus DNA as the substrate (Kunitz unit)
