The CBCU is a component of the ReadyToProcess WAVE 25 bioreactor system. It provides gas to the culture, and monitors pH and/or dissolved oxygen. The compact system contains a controller for mass flow, sensors for gas pressure and O₂ and CO₂ concentrations, and transmitters for pH and DO. An optical fiber cable (Bag Sensor Adaptor 2.5m Assy Item) is delivered with the system.
Three CBCU configurations are available:
• ReadyToProcess CBCU pH: for CO₂, O₂, and pH control
• ReadyToProcess CBCU DO: for CO₂, O₂, and DO control
• ReadyToProcess CBCU Full: for CO₂, O₂, pH and DO control
In single mode, only one ReadyToProcess CBCU is required. When operated in dual mode, culture conditions in the separate Cellbag bioreactors are independently controlled by two ReadyToProcess CBCU units.
CO₂/O₂/air mix controller
Depending on the configuration, air is mixed with CO₂ and/or O₂ according to the setpoints. Nitrogen may be used nstead of air for maintaining a low oxygen environment for nearanaerobic applications.
Gas flow controller
The gas-mix flow is measured and controlled by a mass flow controller (MFC). A correct volumetric flow is achieved by compensation for CO₂ concentration. Quick filling of gas into the Cellbag bioreactor, enabled by the fast-fill function, significantly reduces start-up time. An alarm will inform the user if pressure sensors detect under or overpressure at the gas inlets or within the Cellbag bioreactor.
pH measurement
The pH is measured with optical pH sensors preinstalled in Cellbag bioreactors of pHOPT type. The sensor is connected to the CBCU via an optical fiber cable.
DO measurement
The DO level is measured with optical DO sensors preinstalled in Cellbag bioreactors of DOOPT type. The sensor is connected to the CBCU via an optical fiber cable.
