Our Ready-To-Glow Secreted Luciferase Reporter Assay is a complete system designed for use in a transcription reporter system based on luciferase activity in the supernatant of transfected cells. In the presence of secreted luciferase in the sample, a nonluminescing substrate is converted to a luminescent compound. The intensity of bioluminescence is directly proportional to the amount of luciferase activity. The kit, which includes a substrate buffer, reaction buffer, and a lyophilized secreted luciferase substrate, is available in three reaction sizes. Our Ready-To-Glow Secreted Luciferase Reporter Assay has been customized to work optimally with our Secreted Luciferase Vector Kits.
Features
2–4 fold higher signal than firefly or Renilla luciferase
Single transfection allows multiple time points from same cell
Dual, live-cell assay includes SEAP as well as secreted luciferase:
monitor two promoters, or use one reporter as a normalization control
