Ready-To-Glow™ Dual Secreted Reporter Assay

The Ready-To-Glow Dual Secreted Reporter Assay contains the buffers and substrates for use with the vectors contained in the Ready-To-Glow Dual Secreted Reporter Vector Kit (Cat. No. 631735). This assay allows you to monitor the activity of two promoters simultaneously. The promoters are monitored by testing the media from cells that have been transfected with the two reporter vectors, which express luciferase and SEAP respectively. The assay allows you to monitor promoter activity over time, because samples of the cell media can be taken repeatedly and tested without lysing cells.

Features

• One step assay, for monitoring promoter activity
• 10-fold higher sensitivity then firefly luciferase
• Adaptable from single-tube to high-throughput assays
• Dual, live-cell assay includes secreted luciferase as well as SEAP: monitor two promoters, or use one reporter as a normalization control

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)