Electrophoresis Buffers:
TAE, Tris-Glycine-SDS
Western Blotting, ELISA and Cell Culture:
TBS, TBS-T, PBS, PBS-T
Nucleic Acid Electrophoresis Buffer
QuickSilver™ TAE (Tris-acetate-EDTA)
TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.
TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.
EB1000 QuickSilver TAE Buffer, 1X, 100 packs
Protein Electrophoresis Buffers
QuickSilver™ TGS (Tris-Glycine-SDS)
Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.
EB1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs
Western Blotting, ELISA, Cell Culture, Chromatography
QuickSilver™ PBS and PBST
Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.
EB1200 QuickSilver PBS Buffer, 1X, 100 packs
EB1201 QuickSilver PBST Buffer, 1X, 100 packs
QuickSilver™ TBS and TBST
TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.
EB1202 QuickSilver TBS buffer, 1X, 100 packs
EB1203 QuickSilver TBST buffer, 1X, 100 packs
