pUC 119 DNA

pUC119 is a plasmid vector constructed by inserting a HgiA I(5645) - DraI(5941) fragment containing the intergenic region (IG region) of the M13 phage DNA into the NdeI site of the pUC19 plasmid. Since its multiple cloning site is in the lacZ region, any DNA inserted into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, the cloned DNA can be expressed using the lac promoter.

Features

• Concentration: 0.5 µg/µl
• Form: 10 mM Tris-HCI (pH8.0), 1 mM EDTA
• Chain Length: 3,162 bp

Applications

Cloning of a target gene
DNA sequencing using M13 primers
Determination of long DNA sequences using Deletion Kit for Kilo-Sequencing (Cat. # 6030)
Gene expression using lac promoter

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• pUC118 Vector sequence file in GenBank format