Selection vector which confers hygromycin resistance in mammalian cells for the selection of stably transformed cells. This vector does not contain any enhancer elements and therefore is ideal for use in developing double-stable Tet-Off or Tet-On cell lines because it prevents the unwanted activation of pTRE- and pBI-derived plasmids upon cointegration into the host cell's genome.
Features
Both the linear selection markers and the pTK-Hyg Vector and pPur Vector may be used in cotransfections to establish stable cell lines
Linear selection markers require you to screen fewer clones, resulting in more positive clones than when circular plasmid markers are used
Linear selection markers are ideal for use with all Takara Bio's Tet-On and Tet-Off Inducible Expression Systems—screen fewer candidates and find more highly inducible clones
Applications
Use the Linear Hygromycin Marker or the pTK-Hyg Vector in cotransfections to establish stable cell lines using hygromycin Use the Linear Puromycin Marker or the pPur Vector in cotransfections to establish stable cell lines using puromycin
