pTet-Off Advanced Vector

The pTet-Off Advanced Vector is used to create stable Tet-Off Advanced cell lines as hosts for a Tet-inducible expression system. The Tet-Off Advanced protein is a modified Tet-responsive transactivator (tTA-Advanced) that binds tightly to tetracycline-responsive elements (TRE) and strongly activates transcription of a downstream gene in the absence of tetracycline (Tc) or doxycycline (Dox; a Tc derivative). As Tc or Dox is added to the culture medium, transcription from the TRE or TRE-Tight is turned off in a highly dose-dependent manner.

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• pTet-On Advanced vector information
• pTet-On-Advanced vector sequence file in .gb format