PRP-3 10 µm, 10 x 250 mm
Vendor: Hamilton Company
SKU: 79526
In stock
Hamilton PRP-3 columns are based on the PRP-1 with a larger pore size for the separation of macromolecules such as DNA, RNA oligos, proteins, peptides, and proteomics.. This column resin has no silanol groups, so irreversible protein adsorption is not a concern, giving analytes such as proteins a recovery of > 90%. This stable material also allows the use of concentrated acids, chaotropes, and even detergents to minimize problems with protein solubility.
Application Examples: Organic compounds: large molecules (> 2,000 mw), peptides, proteins, protein digests, protected and deprotected oligonucleotides, nucleic acids
Specifications
| Separation Mode | Reversed-Phase |
|---|---|
| Stationary Phase | PRP-3 |
| Format | HPLC Column |
| Hardware Inner Diameter | 10 mm |
| Hardware Length | 250 mm |
| Particle Size | 10 µm |
| USP Classification | USP L21 |
| Packing Material Type | PSDVB |
| Pore Size | 300 Å |
| Particle Surface Area (m2/g) | 48 |
| Pore Volume (cm3/g) | 0.20 |
| Hardware Material | Stainless Steel |
| Max Pressure | 5,000 psi |
| Mobile Phase Limits | pH 1-13, 0-100% aqueous, organic modifier |
| Buffer Strength | 0.0-0.5N |
| Temp Limits | 5-85°C |
| Restoration | Run a gradient of 100% water to 100% acetonitrile. Repeat three times |
| Compound Classification | Peptides/Proteins |
| UOM | 1 EACH |
Documents
Specifications
- Compound classification Peptides/Proteins
- Format HPLC Column
- Hardware inner diameter 10 mm
- Hardware length 250 mm
- Hardware material Stainless Steel
- Max pressure 5,000 psi
- Packing material type PSDVB
- Particle size 10 µm
- Particle surface area m2 g 48
- Pore volume cm3 g 0.20
- Separation mode Reversed-Phase
- Stationary phase PRP-3
- Sub category Reversed Phase
- Uom 1 EACH
links and documants
- Application note url Click here
- Sds url Click here
- Source url Click here
- Specification url Click here
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PRP-3 10 µm, 10 x 250 mm
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