ProteoTuner™ Shield System C

The ProteoTuner Shield System C utilizes a ligand-dependent destabilization domain (DD-C) and a membrane-permeant stabilizing ligand, Shield1, to quickly and directly regulate the amount of your protein of interest present in a cell or organism. First, your gene of interest is cloned into the pPTunerC Vector and expressed as a fusion with the destabilization domain (DD-C). Then the Shield1 ligand is either added or withheld, depending on your experiment.

Features

• Rapid kinetics: protein level changes in minutes allows accurate functional analysis
• Precise tuning: precise control of protein level by controlling the dose of Shield1
• Reversible control: "protein on" to "protein off" for convincing gene-function studies
• What you get: each kit is supplied with a plasmid vector and an aliquot of Shield1.

Applications

Protein function in pathways
Functional analysis of subunits of a protein complex
Functional analysis of essential proteins

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• ProteoTuner Shield System C Components List
• pPTunerC Vector Information
• pPTunerC vector sequence in GenBank format