Proteinase K is a highly active serine protease. Its enzyme activity is stable in the presence of calcium salts, and increases when a protein denaturant such as SDS or urea is also present. Proteinase K is compatible with a wide range of substrates. It preferentially degrades estel and peptide bands located next to a C-terminal hydrophobic, sulfuric, or aromatic amino acid.
Features
DNA, RNA, and phage purification
Protein digestion
Chromosomal DNA preparation for in situ hybridization, fingerprinting, colony or plaque hybridization, and pulse-field electrophoresis
Applications
DNA, RNA, and phage purification Protein digestion Chromosomal DNA preparation for in situ hybridization, fingerprinting, colony or plaque hybridization, and pulse-field electrophoresis
