Protein G Sepharose Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow resin.
- High binding capacity: approximately 20 mg human IgG per mL resin
- Broad-spectrum: binds to a broad range of IgG species and subclasses
- Complements Protein A resins: unlike protein A, protein G binds all human and mouse IgG subclasses, including human IgG3 and mouse IgG1 as well as all rat IgG subclasses
- No specific albumin binding: recombinant protein G has been genetically engineered to remove the albumin binding region
- Minimal leaching: optimized coupling method minimizes leaching of recombinant protein G and improves binding capacity for IgG
- Wide range of formats: including loose resin packs from 5 mL to 5 L, for manual or automated antibody purification and immunoprecipitation procedures
IgG purification
Protein G Sepharose 4 Fast Flow is a good first choice for general-purpose capture of antibodies at laboratory scale. Compared to protein A-based resins, it binds a broader range of immunoglobulins from eukaryotic species and more classes of IgG.
Key applications include:
- isolation and purification of IgG from serum, ascites, and tissue culture media
- monoclonal antibody purification
- isolation of antibody-ligand immune complexes
- immunoprecipitation protocols
The recombinant protein G ligand binds strongly to the Fc-region from a variety of mammalian species. In addition, many antibodies also interact via the Fab region with a low affinity site on protein G. The ligand is immobilized via a multipoint coupling to the Sepharose 4 Fast Flow agarose matrix to minimize ligand leakage.
Protein G Sepharose 4 Fast Flow resin is part of Cytiva's BioProcess media (resins) range, which ensures security of supply and comprehensive technical and regulatory support.
Successful use in published research
This resin is used in a variety of affinity chromatography applications, including:
Bioprocess applications
Protein G Sepharose Fast Flow resin is used in specific separation applications where broad binding specificity is required. An example is the scavenging of bovine IgG from serum prior to cell culture.
Compatible empty columns – research and bioprocess scale
This Fast Flow affinity chromatography resin can be packed into Cytiva's Tricorn, XK, and HiScale columns.
For process-scale applications, Cytiva recommends AxiChrom columns. Protein G Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. Protein G Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Convenient prepacked columns – research use
This resin is available prepacked into Protein G GraviTrap columns for use with gravity flow.
Other Cytiva products for antibody purification
Life Sciences solutions from Cytiva include a wide array of protein A- and protein G-based resins and columns for use with gravity flow, centrifugation, pumps, or chromatography systems.
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