PrimeScript RT reagent Kit with gDNA Eraser is a reverse-transcription kit for real-time RT-PCR (RT qPCR) that includes a genomic DNA elimination reaction. cDNA synthesis from RNA can be achieved without sample loss in a rapid reaction that is complete in less than 20 minutes. Genomic DNA is eliminated by treatment for 2 minutes at 42°C with gDNA Eraser, which has potent DNA degradation activity. Then a reverse-transcription reaction reagent is added that includes a component which completely inhibits DNA degradation activity, and the reverse-transcription reaction proceeds for 15 minutes. The cDNA obtained using this product can be used with either an intercalating green dye qPCR assay or probe qPCR assay. Please use in combination with quantitative PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A) and Probe qPCR Mix (Cat. #RR391A). Cat. # RR047B contains 4 of Cat. # RR047A. Please refer to Cat. # RR047A for complete product documentation and resources.
Features
Genomic DNA contamination elimination in only 2 minutes
Fast cDNA synthesis—reverse transcription in just 15 minutes
Suitable for use with routine and challenging RNA templates alike
Full-length cDNA synthesis up to 12 kb
Applications
Elimination of genomic DNA contamination
cDNA synthesis for real-time RT-PCR (qPCR)
Two-step real-time RT-PCR Choose the right PrimeScript kit for real-time RT-PCR:
