PrimeScript II 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize 1st strand cDNA from total or poly(A)+ RNA using PrimeScript II RTase. Non-specific binding of reverse transcriptase to long RNA templates and to RNA templates containing complex structures can be a major interfering factor in cDNA synthesis. In addition, non-specific elongation due to mis-priming can have negative effects on RT-PCR and on production of full-length cDNA. PrimeScript II RTase suppreses these harmful effects in the cDNA synthesis reaction. This kit facilitates efficient production of full-length cDNA products, promotes efficient use of polyA, and provides rapid synthesis and reaction times at standard temperature (42°C). Furthermore, suppression of non-specific elongation during reaction assembly allows reactions to be assembled and left on ice for long periods of time before initiating reverse transcription. First strand cDNAs synthesized with this kit can be used for a variety of applications including second strand synthesis, hybridization, and PCR amplification. Furthermore, this kit is useful in any application that requires high-quality, full-length cDNA.
