The PrimeScript 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize first strand cDNA from total or polyA+ RNA using PrimeScript RTase, a MMLV (Moloney Murine Leukemia Virus)-derived reverse transcriptase. This enzyme is capable of synthesizing cDNA up to 12 kb in length. PrimeScript RTase can synthesize cDNA efficiently at 42℃, even from RNA templates that contain GC-rich regions or complex secondary structures, making it unnecessary to perform reactions at high temperatures that may degrade RNA. The first strand cDNA synthesized with this kit can be used for variety of applications, including second strand synthesis, hybridization, PCR amplification, and real-time PCR.
