pRI 909/910 DNA are binary vectors that have a region of T-DNA for plant transformation. This originated from Ri plasmid of Rhizobium (Agrobacterium tumefaciens) rhizogenes, lacking a vir region. pRI 909/910 DNA are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are propagated at high copy number due to the presence of ColE1 ori. pR1909/910 are maintained stably in Rhizobium (Agrobacterium) due to mutant type replication origin of Ri plasmid (Ri-ori). These vectors include NPTIII to confer Kanamycin resistance as the selection marker for E. coli and Rhizobium (Agrobacterium), a mutant type kanamycin resistant gene NPTII as the selection marker for plant, and the same multi-cloning sites as pUC type plasmids. The vectors are available for Agrobacterium-mediated binary plant transformation. Stable chromosomal integration of a target gene is possible because the cloning site is located at the position closer to Right Border (RB) of T-DNA than the selection marker (NPT II) for the plant, so the target gene is not deleted.
Features
Easy and stable plant chromosome target gene integration
