Use pRI 201-AN DNA to transform dicotyledonous plants. Use pRI 201-ON DNA to transform monocotyledonous plants. The presence of 2 multicloning sites enable multigene transformation with a single vector. Vectors contain a replication origin (ColE1 ori) derived from pUC plasmids, which allows maintenance at a high-copy- number in E. coli. pRI 201 DNA series are binary vectors designed for expressing target genes in transformed plant cells. This series of vectors retain the backbone of pRI 101 vectors (Cat.# 3262/3263), which includes an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5' -UTR) (translational enhancer region) downstream of the 35S promoter from cauliflower mosaic virus (CaMV). In addition, they have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI 101 series 2. Further, multigene transformation with a single vector is possible by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the cloning site (MCS2) downstream of the HSP terminator. The pRI 201 series has two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. Carrying an Arabidopsis ADH-derived 5' -UTR (AtADH 5' -UTR), pRI 201-AN DNA is suitable for dicotyledonous plants. With a rice ADH-derived 5' -UTR (OsADH 5' -UTR), pRI 201-ON DNA is compatible with monocotyledonous plants.
Features
Use pRI 201-AN DNA to transform dicotyledonous plant species
Use pRI 201-ON DNA to transform monocotyledonous plant species
Integrate multiple transgenes using a single vector
High copy number (ColE1 ori) replication in E. coli
