pRI 101-ON DNA

pRI 101 DNA series are binary vectors for expressing foreign genes in plant cells, which include the 35S promoter of cauliflower mosaic virus (CaMV) and a 5' noncoding region (5'-UTR) of alcohol dehydrogenase (ADH) gene. The 5'-UTR of ADH functions as a translational enhancer in plants. There are two types of vectors, pRI 101-AN DNA having a 5'-UTR of Arabidopsis ADH (AtADH 5'-UTR) and pRI 101 ON DNA having a 5'-UTR of rice ADH (OsADH 5'-UTR). The pRI 101-AN is for dicotyledonous plants such as tobacco or Arabidopsis and the pRI 101-ON is for monocotyledonous plants such as rice. The pRI 101 DNA are shuttle vectors, and replicate autonomously in E. coli and Rhizobium (Agrobacterium).

Features

• Agrobacterium-mediated plant transformation
• Easy and stable integration of dicot and monocot target genes into plant chromosomes
• Increased target gene expression via the 5'-UTR translation enhancer region
• Vector versatility for dicotyledonous and monocotyledonous plant cells

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• pRI 101-AN DNA Vector sequence file in GenBank format