Premix Ex Taq (Perfect Real Time) is designed for probe-based qPCR, and is also suitable for fast PCR. Premix Ex Taq allows accurate target quantification and detection over a broad dynamic range and makes it possible to obtain highly reproducible and reliable realtime PCR results. The product is supplied as a 2X premix to facilitate easy preparation of reaction mixtures. The combination of Takara Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, and a buffer optimized for real-time PCR suppresses non-specific amplification and allows high amplification efficiency and high detection sensitivity in real-time PCR analyses.
Features
High specificity and increased amplification efficiency for qPCR
Sensitivity down to 10 copies for intercalating green dye or probe detection
Compatibility with 5' nuclease assays and with TB Green dye
Compatibility with many commonly used real-time PCR instruments
Applications
Real-time PCR using probe-based qPCR assays or intercalating green dye
