PreDictor™ Capto™ Butyl plate

These 96-well filter plates are prefilled with Capto™ Butyl hydrophobic interaction chromatography (HIC) resin for use in high-throughput process development workflows.

• Allows parallel screening of chromatographic conditions
• Allows fast and efficient evaluation of parameters for binding, wash, and elution conditions.
• Prefilled with Capto™ Butyl HIC resin.
• Can be used manually or with automated robotic systems.

About PreDictor™ plates for high-throughput process development

PreDictor™ plates are disposable, 96-well filter plates for parallel screening of resins and chromatographic conditions.

Data generated using PreDictor™ plates show good correlation with data from chromatography columns, making the plates an excellent tool for initial screening. The plates can be used with a robotic system or operated manually with a multichannel pipet (using vacuum or centrifugation), as shown in this video.

Learn how our PreDictor™ plates can be used for developing HIC steps:

• Step-by-step HTPD workflow to optimize a HIC step
• Developing a HIC polishing step for removal of mAb aggregates

Be sure to download Assist software to support the PreDictor™ plate workflow, from setup of experimental design to data evaluation.

Learn more about HTPD.

Capto™ Butyl – a high-flow and high-capacity HIC resin

Capto™ Butyl hydrophobic interaction chromatography (HIC) resin with a butyl ligand used in the capture and intermediate purification of mAbs, mAb variants, and other biomolecules. It combines high capacity, narrow specification range, high flow rate, and low backpressure to meet the demands of large-scale biopharmaceutical manufacturing today.

Not sure which HIC resin you should use? We recommend starting with one of the kits below:

• PreDictor™ Capto™ HIC screening kit a set of 96-well plates prefilled with six Capto™ hydrophobic interaction chromatography resins for use in high-throughput process development (HTPD).
• HiTrap™ Capto™ HIC selection kit, a set of five Capto™ HIC resins in HiTrap™ columns for resin selection and condition screening.

Capto™ Butyl is available in other formats (Fig 1):

• Bulk resin bottles and containers of Capto™ Butyl resin for column packing.
• PreDictor™ Capto™ Butyl RoboColumn™ units, miniaturized columns for HTPD.
• HiTrap™ Capto™ Butyl 1 mL and 5 mL columns for easy use with a syringe, peristaltic pump, or chromatography system.
• HiScreen™ Capto™ Butyl columns optimized for method and process development.
• ReadyToProcess™ Capto™ Butyl columns, in various sizes for scale-up and GMP manufacturing.

Fig 1. Prepacked formats from Cytiva, to support various scales, from screening to GMP manufacturing.

Documents

• Data file (PDF)
• Instructions for use (PDF)