Ponceau S is used for the reversible staining of proteins blotted on nitrocellulose or PVDF membranes. The reversibility of the staining process is ideally suited for subsequent immunoblots (western blot) or other color reactions as Ponceau S can be completely removed. For staining, the membrane (nitrocellulose or PVDF) is incubated in a 0.1% – 0.5% Ponceau S solution in 5% acetic acid for 5 – 10 minutes. Incubating the stained membrane in 5% acetic acid for 15 minutes to destaining.
Directions for Use
Add the Ponceau S solution to the membrane incubate for 5-10 min and monitor the color development.
Destaining is done by incubating the stained membrane in 5% acetic acid for 15 minutes, the membrane can then be immunologically detected by Western blot.
Storage Conditions
Supplied as ready-to-use solution, 500mL Shipping, and store at RT.
