pLVX-EF1α-IRES-ZsGreen1 is a bicistronic lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector contains an internal ribosomal entry site (IRES) that allows a gene-of-interest and the ZsGreen1 fluorescent protein to be simultaneously coexpressed from a single mRNA transcript. Expression of the transcript is driven by the constitutively active human elongation factor 1 alpha (EF1α) promoter.
Features
High-titer lentiviral vector system
Constitutive expression from the human EF1 alpha promoter
Robust, long-term expression, in the broadest range of cell types
Bright fluorescent proteins to monitor expression or localization, learn more from our fluorescent protein quick guide.
Applications
If CMV promoters are silenced in your cell type, try EF-1 alpha Lentiviral transduction of stem cells Lentiviral transduction of hematopoietic cells
