pLVX-EF1α-DsRed-Monomer-N1 is a lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector allows a gene-of-interest to be fused to the N-terminus of the fluorescent protein DsRed-Monomer. Expression of the fusion is driven by the human elongation factor 1 alpha (EF1α) promoter, which continues to be constitutively active even after stable integration of the vector into the host cell genome. Stable expression of the fusion allows the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells), without the transgene silencing associated with CMV promoters. In addition, the vector allows efficient flow cytometric detection of stably or transiently transfected mammalian cells expressing DsRed-Monomer fusions, without time-consuming drug and clonal selection.
Features
High-titer lentiviral vector system
Constitutive expression from the human EF1 alpha promoter
Robust, long-term expression, in the broadest range of cell types
Bright fluorescent proteins to monitor expression or localization, learn more from our fluorescent protein quick guide.
Applications
If CMV promoters are silenced in your cell type, try EF-1 alpha Lentiviral transduction of stem cells Lentiviral transduction of hematopoietic cells
