pIRES2-ZsGreen1 is a bicistronic expression vector containing ZsGreen1 as a marker for transfection efficiency. It encodes a human-codon-optimized variant of the Zoanthus sp. green fluorescent protein, ZsGreen, which has been engineered for brighter fluorescence and higher expression in mammalian cells. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES). The IRES sequence allows the gene of interest and ZsGreen1 to be translated simultaneously from the same mRNA transcript.
Features
Exceptional brightness: up to 4X brighter than EGFP
ZsGreen1 excitation and emission maxima are 493 nm and 505 nm, respectively
We offer both monoclonal and polyclonal ZsGreen1 antibodies, which recognize native and denatured forms of ZsGreen1 fluorescent protein, as well as fusion proteins containing ZsGreen1. They do not cross-react with Aequorea sp. GFPs (AcGFP1, EGFP).
Bicistronic (pIRES-) expression vectors are available
Lentiviral (pLVX-) and retroviral (pRetroX-) formats are available
