TriboTM Plasmid DNA Rapidprep Mini Kit provides a fast, simple, and cost-effective plasmid DNA preparation method. The main feature of the kit is that it uses a novel technology to combine clear filter and mini column into one set to combine the lysate clearance and the plasmid DNA binding into one step (Fig.1). This unique modification dramatically increases the purity and yield rate of plasmid and shortens 50% of the processing time in comparison to current similar products in the market. The procedure can be finished in 10 minutes.
This kit can be ideally used to isolate and purify plasmids less than 10 kb. The purification yield is up to 30 µg of plasmid DNA. Efficiency may be reduced as the size increases.
Key Features
Rapid: 10 minutes or 50% time will be saved compared to similar products in the market.
Unique: Assemble the Clear Filter column and the DNA Mini Column together to make the lysate clearance and plasmid DNA binding into 1 step.
High purity and yields: The kit utilizes high binding Column D to increase the purity and yields of the plasmid.
Kit Contents
Part
TBS6011-50
TBS6011-200
Clear Filter Column (small one)
50
200
DNA Mini Column (includes Column D & Collection Tube)
50
200
Buffer 1 (B1)
20mL
60mL
Buffer 2 (B2)
20mL
60mL
Buffer 3 (B3)
25mL
90mL
Buffer 4 (B4)
19mL
69mL
Buffer 5 (B5)
12mL
50mL
Buffer 6 (B6)
15mL
30mL
RNase A (20 mg/mL)
0.1mL
0.3mL
Storage Conditions
Store at room temperature for shelf life of 12 months after receipt. The buffer 1 (B1) with RNase A is suggested at 4°C.
Castel, P., Holtz-Morris, A., Kwon, Y., Suter, B. P., & McCormick, F. (2021). DoMY-Seq: A yeast two-hybrid-based technique for precision mapping of protein-protein interaction motifs. The Journal of biological chemistry, 296, 100023. https://doi.org/10.1074/jbc.RA120.014284
