Bicistronic expression vector for the simultaneous translation of two genes of interest from the same mRNA transcript. Each gene should be inserted into one of two MCSs located on either side of the EMCV internal ribosomal entry site (IRES). The entire cassette is driven by the CMV promoter. The vector also contains the neomycin antibiotic resistance gene for selection with G418 in mammalian cells.
Features
Express your protein of interest and an antibiotic resistance marker or fluorescent protein from a single mRNA
Quickly identify cells expressing your protein of interest at high levels
Bicistronic expression allows faster, more stable clone selection
Choice of vectors enables screening via antibiotic selection, fluorescence microscopy, or flow cytometry
pLVX vectors are lentiviral vectors—a lentiviral packaging system is required to make lentiviral particles from these constructs.
pLVX vectors are lentiviral vectors—a lentiviral packaging system is required to make lentiviral particles from these constructs.
Applications
Rapidly select stable clones that show high-level expression of your target protein Identify transfected cells by antibiotic selection, fluorescence microscopy, or flow cytometry
