PicoPLEX® Single Cell WGA Kit v3

The PicoPLEX Single Cell WGA Kit v3 uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by high-fidelity amplification with very low background to yield over 2 µg of product in under 3 hours. This kit is suited for applications in which base-level resolution is required, such as for the detection of mutations. This product contains reagents for 96 reactions.

Features

• Whole-genome amplification directly from 1–10 cells or genomic DNA equivalents
• Industry-leading reproducibility and coverage uniformity for robust detection of CNVs using NGS, array, or qPCR-based approaches
• Increased fidelity and reduced allele dropout rates for improved SNV analysis (PicoPLEX v3)
• Can be paired with commonly used NGS library preparation methods and target enrichment platforms
• Also available as a combined workflow for WGA and NGS library prep

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• Components
• Components