pGBKT7 DNA-BD Vector

pGBKT7 is a yeast expression vector that is designed to express a fusion protein of the GAL4 DNA-binding domain (DNA-BD; amino acids 1–147) and a bait protein. The fusion protein is expressed at high levels from the medium ADH1 promoter. The fusion protein also contains a c-Myc epitope tag. To facilitate in vitro transcription/translation, pGBKT7 includes the T7 promoter between the GAL4 DNA-BD and the epitope tag.

Features

• Acting as a bridge, by interacting with two proteins that do not directly interact with each other
• Stabilizing a weak interaction between the two proteins
• Modifying or inhibiting one or both of the proteins (Osbourne et al 1995)
• Inhibiting the two-hybrid interaction

Applications

Yeast two-hybrid screening
Yeast three-hybrid screening

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• pGADT7 AD Vector Information
• pGADT7 AD Vector Sequence in GenBank Format