pET Express & Purify Kit—His60 (In-Fusion® Ready)

The pET Express & Purify Kit-His60 (In-Fusion Ready) allows you to use In-Fusion cloning technology to express a protein of interest with an N- or C-terminal 6xHN tag in E. coli. The system contains IPTG-inducible, pET-based bacterial expression vectors that have been prelinearized to allow In-Fusion cloning via the included In-Fusion HD Enzyme Premix. These vectors contain a T7/lac promoter and polyhistidine tag sequence for high-level expression of his-tagged proteins, which can be easily purified with the high-capacity His60 Ni Gravity Columns included in the kit. The kit also contains a control vector that expresses an N-terminal, 6xHN-tagged, GFPuv fusion protein.

Features

• No copurification of unwanted host proteins
• No SlyD contamination (histidine-rich protein present in E. coli)
• Lowest metal ion leakage
• Available in various formats as bulk resin, cartridges, and gravity columns

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• pET Express & Purify Kit--His60 (In-Fusion Ready) Components List
• pET6xHN-C (In-Fusion Ready) vector sequence file in .gb format
• pET6xHN-N (In-Fusion Ready) vector sequence file in .gb format
• Components
• Components
• Components