Preparation and Specification
Appearance: Reddish-brown amorphous powder, lyophilized
Activity: GradeⅠ 250 Purpurogallin U/mg-solid or more (-131) (RZ ≥ 3.0, salt free)
GradeⅢ 110 Purpurogallin U/mg-solid or more (-301) (RZ ≥ 2.0, containing approx. 30% of stabilizers)
GradeⅢ 180 Purpurogallin U/mg-solid or more (-302) (RZ ≥ 2.0, salt free)
Contaminant: Phosphatase ≤1.0×10⁻³% (GradeⅢ)
Properties
Stability: Stable at -20°C for at least Two years
Molecular weight: approx. 40,000
Structure: Glycoprotein with one mole of protohaemin IX
Inhibitors: Cyanide, sulfide, fluoride, azide
Optimum pH: 6.0-7.0
Optimum temperature: 45°C
pH Stability: pH 5.0-10.0 (25°C, 20hr)
Thermal stability: below 50°C (pH 6.0, 10min)
Applications
This enzyme is useful for enzymatic determination of H₂O₂ in clinical analysis. Especially, the highly purified preparation (Grade I) is useful as a protein tracer in histo-and cyto-chemistry and as a valuable experimental tool in hodological neurography. Also, the enzyme preparation has been used as an enzyme label in enzyme immunoassay. Grade III is suitable for dry chemistry. On the other hand, the enzymes contribute for the reduction of phehol in waste water.
Research use only.
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