pEF1α-IRES-ZsGreen1 is a bicistronic mammalian expression vector that allows the simultaneous, constitutive expression of a protein of interest and the green fluorescent protein ZsGreen1. Constitutive expression of the bicistronic transcript is driven by the human elongation factor 1 alpha (EF1α) promoter.
Features
Exceptional brightness: up to 4X brighter than EGFP
ZsGreen1 excitation and emission maxima are 493 nm and 505 nm, respectively
We offer both monoclonal and polyclonal ZsGreen1 antibodies, which recognize native and denatured forms of ZsGreen1 fluorescent protein, as well as fusion proteins containing ZsGreen1. They do not cross-react with Aequorea sp. GFPs (AcGFP1, EGFP).
Bicistronic (pIRES-) expression vectors are available
Lentiviral (pLVX-) and retroviral (pRetroX-) formats are available