pEF1α-HA is a mammalian expression vector that constitutively expresses a protein of interest fused to an N-terminal hemagglutinin (HA) epitope tag, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the tagged protein is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the fusion to be expressed without the transgene silencing associated with CMV promoters.
Features
Co-immunoprecipitation of myc-tagged and HA-tagged proteins in mammalian cells
Strong constitutive expression
Confirm yeast two-hybrid interactions in mammalian cells
Strong, clean bands via Western blot
Applications
Co-immunoprecipitation studies Confirmation of protein-protein interactions Western blotting Epitope-tagged expression
