pDsRed-Monomer-Hyg-N1 Vector

pDsRed-Monomer-Hyg-N1 encodes DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed. pDsRed-Monomer-Hyg-N1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to DsRed-Monomer. The vector contains a hygromycin resistance gene for the selection of stable transfectants. pDsRed-Monomer-Hyg-N1 can also be used as a cotransfection marker since the unmodified vector constitutively expresses DsRed-Monomer. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Features

• Truly monomeric 28-kDa protein confirmed by FPLC gel filtration chromatography and pseudonative gel electrophoresis
• DsRed-Monomer excitation and emission maxima are 556 and 586 nm, respectively
• Detect 12 hr after transfection
• Highly stable protein

Applications

Ideal for creating fluorescent fusion proteins
Subcellular localization studies
Dual labeling with AcGFP1
Stable cell lines

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• pLVX-EF1alpha-DsRed-Monomer-N1 Vector Information
• pLVX-EF1a-DsRed-Monomer-N1 vector sequence in GenBank format