pBI-CMV1 is a mammalian bidirectional expression vector that allows the constitutive expression of two proteins of interest. Each gene must be cloned into one of the two independent multiple cloning sites (MCS 1 and MCS 2), and contain both an initiation codon and a stop codon. Protein expression is driven by one of two constitutively active human cytomegalovirus promoters, PCMV IE and PminCMV, located just upstream of MCS 1 and MCS 2, respectively.
Features
Constitutively express a protein of interest and a reporter protein (or two proteins of interest)
pBI-CMV1 has two cloning sites and allows the constitutive expression of two proteins of interest.
pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
pBI-CMV3 allows you to express a protein of interest and ZsGreen1, an extremely bright green fluorescent protein derived from Zoanthus sp. reef coral.
pBI-CMV4 allows you to express a protein of interest and DsRed2, a red fluorescent protein derived from Discosoma sp. reef coral.