pAcGFP1-Actin Vector

pAcGFP1-Actin encodes a fusion protein consisting of Aequorea coerulescens green fluorescent protein (AcGFP1) fused at its C-terminus to the human cytoplasmic b-actin. The AcGFP1-Actin fusion protein incorporates into growing actin filaments, allowing visualization of the actin cytoskeleton in living or fixed cells.

Features

• Monomeric green fluorescent protein
• Similar spectral properties to EGFP
• AcGFP1 excitation and emission maxima: 475 and 505 nm, respectively
• EGFP excitation and emission maxima: 484 and 510 nm, respectively
• Lentiviral (pLVX-) and retroviral (pRetroQ-) formats are available

Applications

Fusions (N or C terminus)
Transfection controls
Dual labeling with DsRed-Monomer fluorescent protein or mCherry fluorescent protein
Bicistronic coexpression with another protein of interest (pIRES- vectors) 
Subcellular localization studies

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• pAcGFP1-Mito vector information
• pAcGFP1-Mito vector sequence in GenBank format