pAAV-ZsGreen1 Vector

pAAV-ZsGreen1 Vector is an adeno-associated virus (AAV) vector plasmid that has terminal hairpin structures called inverted terminal repeats (ITRs) at both ends, which function as origins of genomic replication and contribute to packaging of viral particles. This vector plasmid allows you to produce high titer recombinant AAV vector together with AAVpro Helper Free System (AAV2) (Cat. #6230). It carries the ZsGreen1 gene, a variant of the reef coral Zoanthus sp. green fluorescent protein, and allows ZsGreen1 to be used as an indicator of transfection efficiency and transduction efficiency.

Features

• An AAV vector that contains ZsGreen1 under the control of a CMV promoter, and Inverted Terminal Repeats (ITRs)
• Expression of ZsGreen1 can be used to assess transfection efficiency during AAV particle preparation and the titer of prepared particles
• ZsGreen1 is an exceptionally bright green fluorescent protein, 2.5X brighter than EGFP
• Learn more about ZsGreen1 from our fluorescent protein quick guide

Applications

A positive control for monitoring AAV particle preparation with AAVpro Helper Free Systems

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• pAAV-ZsGreen1 vector sequence in .fasta format