NSC-23766, Rac1 GTPase inhibitor

Product Details

Formal Name: N6-[2-[[4-(Diethylamino)-1-methylbutyl]amino]-6-methyl-4-pyrimidinyl]-2-methyl-4,6-quinolinediamine trihydrochloride

Molecular Formula: C₂₄H₃₅N₇ · 3HCl

Formula Weight: 531.00

CAS Number: 733767-34-5

Purity: >98%

Formulation: powder

Solubility: Soluble in DMSO (up to 50 mg/ml) or in Water (up to 50 mg/ml).

Storage: RT

Stability: ≥ 1 year.

Applications

Rac1 GTPase inhibitor

Functions

Selective inhibitor of Rac1-GEF interaction. Prevents Rac1 activation by Rac-specific guanine nucleotide exchange factors (GEFs) Trio and Tiam1 (IC50 ca. 50 μM) with no effect on the closely related Cdc42 or RhoA. In cells, it blocks serum or PDGF-induced Rac-1 activation and lamellipodia formation. NSC-23766 suppressed the growth of wild-type NIH 3T3 cells but was inactive in cells expressing the constitutively active Rac1 mutant, L61Rac1. It is a useful tool to probe the involvement of Rac1 in cell signalling. Off target effect: antagonist at muscarinic acetylcholine receptors. Cell permeable.

Application Procedures

First dissolved in DMSO (up to 50 mg/ml) or in Water (up to 50 mg/ml), then diluted to aqueous buffer. Solutions in DMSO or distilled water may be stored at -20°C for up to 3 months.

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)