NovaSeq X Plus Sequencing System

Built on decades of innovation from a trusted partner in genomics, the NovaSeq X Series will enable your most ambitious ultra-high-throughput projects. These are our most powerful sequencers yet. Scale your studies with three flow cell types and 16–21 Tb output per run on the dual flow cell NovaSeq X Plus System or 8–10.5 Tb on the single flow cell NovaSeq X System.* Ultra-high-density flow cells and ultra-high-resolution optics enable output of 26–35 billion single reads per flow cell.

Extraordinary throughput and accuracy to perform data-intensive applications at production scale

The NovaSeq X Series is powered by XLEAP-SBS chemistry — an even faster, higher quality, and more robust version of our proven sequencing by synthesis (SBS) chemistry

XLEAP-SBS chemistry delivers the highest level of data accuracy and performance, and the increased stability enables remarkable sustainability benefits.

Flexibility to run DRAGEN analysis your way, either onboard, in the cloud, or on-server

Access a broader menu of applications through cloud workflows on Illumina Connected Analytics or BaseSpace Sequence Hub.

The innovations built into the NovaSeq X and NovaSeq X Plus Systems have the power to generate tens of thousands of whole genomes per year.

Dream big. NovaSeq X Series can make it happen.

Transformative economics for transformative breakthroughs

The NovaSeq X Series allows you to maximize your productivity and minimize your turnaround time. Beyond the significant reduction in cost per Gb and per read, the NovaSeq X series incorporates cost-efficiency throughout the workflow. Streamlined informatics, operational simplicity, sustainability advancements, and world-class support lead to the best total cost of ownership equation.

Propel your research forward to impact the world, not the environment. The NovaSeq X and NovaSeq X Plus Systems were purpose-designed to be our most sustainable sequencing systems yet.

A new standard in operational simplicity

More results, fewer steps, less effort. The NovaSeq X Series workflow includes flexible run setup, automated ultra-high-throughput sequencing with onboard cluster generation, accelerated data analysis with DRAGEN platform onboard, and more.

Unimaginable experiments, made unbelievably cost-efficient

The NovaSeq X Series helps moonshot projects become a reality—making affordable what was previously out of reach. Increase statistical power with broader study designs and larger sample cohorts. Maximize read numbers and increase sequencing depth for a higher resolution view that can detect low-frequency signals and variants.

The NovaSeq X Plus System revolutionizes human whole-genome sequencing, with the ability to sequence more than 128 genomes per run. These production-scale sequencing systems can also perform a broad range of data-intensive applications, from single-cell analysis to exome and circulating tumor DNA sequencing, that will unlock the next wave of omics discovery.

Key Features

• Push-button operation with automated onboard cluster generation and post-run wash
• Individually addressable flow cell lanes with automated onboard independent lane loading
• Flexible run planning options, touchless secondary analysis workflows, and automated lossless data compression
• Thoughtful ergonomic design innovations for the ultimate user experience
• Save time and money with a 90% reduction in packaging weight and waste
• Maximize storage space with compact cartridges and packaging
• Save disposal costs with no dry ice or ice packs
• Manage workflow: Track samples and manage your runs with manual or automated LIMS options.
• Prepare libraries: Access a broad range of high-performance library preparation kits.
• Sequence: Use XLEAP-SBS chemistry—advanced reagents built upon proven Illumina SBS—to power faster, more accurate sequencing.
• Analyze data: Leverage DRAGEN platform onboard or in the cloud for accurate, rapid, secondary data analysis.
• Interpret results: Interpret results, get insights, and drive discovery with cloud connection to tertiary analysis tools.
• Powered by XLEAP-SBS chemistry: The NovaSeq X Series is powered by XLEAP-SBS chemistry, our fastest, highest quality, and most robust sequencing by synthesis (SBS) chemistry to date. Built upon the proven foundation of standard Illumina SBS chemistry, XLEAP-SBS chemistry delivers improved reagent stability with two-fold faster incorporation times.
• Ultra-high density patterned flow cells: The NovaSeq X Series incorporates patterned flow cell technology to generate an ultra-high level of throughput for a broad range of sequencing applications. NovaSeq X patterned flow cells contain tens of billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.
• Streamlined, comprehensive informatics: The NovaSeq X and NovaSeq X Plus Systems are integrated with the DRAGEN secondary analysis platform for ultra-rapid, accurate genomic data analysis. Run multiple secondary analysis pipelines in parallel, with data seamlessly analyzed anywhere within the DRAGEN ecosystem. Run up to four simultaneous applications per flow cell in a single run.
• Genomic data compression: Integrated DRAGEN ORA (original read archive) can perform lossless compression to reduce FASTQ file sizes by up to five-fold. This optimized genomics data compression enables faster data transfers and easier data management. The smaller data footprint also reduces storage and energy consumption costs.
• More discoveries, more sustainably than ever: The NovaSeq X Series was purpose-built for improved sustainability. Innovations include lyophilized reagents that arrive at room temperature and a ~90% reduction in packaging waste for reagents. Plastic mass is reduced by over 50% and the buffer cartridge is made with a plant-based biopolymer.
• Ultra-high-resolution optics: The NovaSeq X Series achieves ultra-high-resolution imaging through higher numerical aperture, a custom CMOS sensor, and two-channel SBS with blue-green optics. The smaller wavelengths allow for tighter spacing between nanowells for 26–35 billion single reads on NovaSeq X Series 25B flow cells.

Applications

• Human genomes
• Exomes
• Transcriptomes

Specifications

NovaSeq X Series specifications
Output range	~165 Gb–21 Tb
Reads per run	~1.6–52 billion
Max read length	2 × 300 bp
Run time	~14–48 hr
NovaSeq X Plus
Maximum output per run	~16–21 Tb
NovaSeq X
Maximum output per run	~8–10.5 Tb
NovaSeq 6000
Maximum output per run	6 Tb
NovaSeq X Plus
Maximum reads per run	52–70 billion single reads; 104–140 billion paired-end reads
NovaSeq X
Maximum reads per run	26–35 billion single reads; 52–70 billion paired-end reads
NovaSeq 6000
Maximum reads per run	20 billion single reads; 40 billion paired-end reads
Output per flow cell for various read lengths — 1.5B
2 × 50 bp	~165–238 Gb
Output per flow cell for various read lengths — 5B
2 × 50 bp	~500–650 Gb
Output per flow cell for various read lengths — 10B
2 × 50 bp	~1–1.3 Tb
Output per flow cell for various read lengths — 25B
2 × 50 bp	~2.6–3.5 Tb
Output per flow cell for various read lengths — 1.5B
2 × 100 bp	~330–476 Gb
Output per flow cell for various read lengths — 5B
2 × 100 bp	~1–1.3 Tb
Output per flow cell for various read lengths — 10B
2 × 100 bp	~2–2.7 Tb
Output per flow cell for various read lengths — 25B
2 × 100 bp	~5.3–7 Tb
Output per flow cell for various read lengths — 1.5B
2 × 150 bp	~500–716 Gb
Output per flow cell for various read lengths — 5B
2 × 150 bp	~1.5–2 Tb
Output per flow cell for various read lengths — 10B
2 × 150 bp	~3–4 Tb
Output per flow cell for various read lengths — 25B
2 × 150 bp	~8–10.5 Tb
Output per flow cell for various read lengths — 1.5B
2 × 300 bp	~1–1.4 Tb
Output per flow cell for various read lengths — 5B
2 × 300 bp	-
Output per flow cell for various read lengths — 10B
2 × 300 bp	-
Output per flow cell for various read lengths — 25B
2 × 300 bp	-
Reads passing filter per flow cell — 1.5B
Single-end reads	~1.6–2.4 billion
Reads passing filter per flow cell — 5B
Single-end reads	~5–6.5 billion
Reads passing filter per flow cell — 10B
Single-end reads	~10–13 billion
Reads passing filter per flow cell — 25B
Single-end reads	26–35 billion
Reads passing filter per flow cell — 1.5B
Paired-end reads	~3.2–4.8 billion
Reads passing filter per flow cell — 5B
Paired-end reads	~10–13 billion
Reads passing filter per flow cell — 10B
Paired-end reads	~20–26 billion
Reads passing filter per flow cell — 25B
Paired-end reads	~52–70 billion
Quality scores and run time — 1.5B
Quality Scores – 2 × 50 bp	≥ 90% of bases higher than Q30
Quality scores and run time — 5B
Quality Scores – 2 × 50 bp	≥ 90% of bases higher than Q30
Quality scores and run time — 10B
Quality Scores – 2 × 50 bp	≥ 90% of bases higher than Q30
Quality scores and run time — 25B
Quality Scores – 2 × 50 bp	≥ 90% of bases higher than Q30
Quality scores and run time — 1.5B
Quality Scores – 2 × 100 bp	≥ 85% of bases higher than Q30
Quality scores and run time — 5B
Quality Scores – 2 × 100 bp	≥ 85% of bases higher than Q30
Quality scores and run time — 10B
Quality Scores – 2 × 100 bp	≥ 85% of bases higher than Q30
Quality scores and run time — 25B
Quality Scores – 2 × 100 bp	≥ 85% of bases higher than Q30
Quality scores and run time — 1.5B
Quality Scores – 2 × 150 bp	≥ 85% of bases higher than Q30
Quality scores and run time — 5B
Quality Scores – 2 × 150 bp	≥ 85% of bases higher than Q30
Quality scores and run time — 10B
Quality Scores – 2 × 150 bp	≥ 85% of bases higher than Q30
Quality scores and run time — 25B
Quality Scores – 2 × 150 bp	≥ 85% of bases higher than Q30
Quality scores and run time — 1.5B
Quality Scores – 2 × 300 bp	≥ 75% of bases higher than Q30
Quality scores and run time — 5B
Quality Scores – 2 × 300 bp	-
Quality scores and run time — 10B
Quality Scores – 2 × 300 bp	-
Quality scores and run time — 25B
Quality Scores – 2 × 300 bp	-
Quality scores and run time — 1.5B
Run Time – 2 × 50 bp	~17 hr
Quality scores and run time — 5B
Run Time – 2 × 50 bp	~14 hr
Quality scores and run time — 10B
Run Time – 2 × 50 bp	~18 hr
Quality scores and run time — 25B
Run Time – 2 × 50 bp	~25 hr
Quality scores and run time — 1.5B
Run Time – 2 × 100 bp	~20 hr
Quality scores and run time — 5B
Run Time – 2 × 100 bp	~18 hr
Quality scores and run time — 10B
Run Time – 2 × 100 bp	~22 hr
Quality scores and run time — 25B
Run Time – 2 × 100 bp	~38 hr
Quality scores and run time — 1.5B
Run Time – 2 × 150 bp	~23 hr
Quality scores and run time — 5B
Run Time – 2 × 150 bp	~22 hr
Quality scores and run time — 10B
Run Time – 2 × 150 bp	~25 hr
Quality scores and run time — 25B
Run Time – 2 × 150 bp	~48 hr
Quality scores and run time — 1.5B
Run Time – 2 × 300 bp	~44 hr
Quality scores and run time — 5B
Run Time – 2 × 300 bp	-
Quality scores and run time — 10B
Run Time – 2 × 300 bp	-
Quality scores and run time — 25B
Run Time – 2 × 300 bp	-
Estimated sample throughput for key applications — 1.5B
Human genomes per flow cell	~4
Estimated sample throughput for key applications — 5B
Human genomes per flow cell	~12
Estimated sample throughput for key applications — 10B
Human genomes per flow cell	~24
Estimated sample throughput for key applications — 25B
Human genomes per flow cell	~64
Estimated sample throughput for key applications — 1.5B
Exomes per flow cell	~27
Estimated sample throughput for key applications — 5B
Exomes per flow cell	~84
Estimated sample throughput for key applications — 10B
Exomes per flow cell	~167
Estimated sample throughput for key applications — 25B
Exomes per flow cell	~436
Estimated sample throughput for key applications — 1.5B
Transcriptomes per flow cell	~30
Estimated sample throughput for key applications — 5B
Transcriptomes per flow cell	~100
Estimated sample throughput for key applications — 10B
Transcriptomes per flow cell	~200
Estimated sample throughput for key applications — 25B
Transcriptomes per flow cell	~520
Instrument specifications
Laser wavelengths	532 nm, 457 nm, 655 nm
Dimension with monitor W × D × H	86.4 cm × 93.3 cm × 158.8 cm (34 in × 36.7 in × 62.5 in)
Dry weight of instrument (not including UPS; no core consumables)	1171 lb
Dry weight of instrument (including UPS; no core consumables)	1253 lb
Wet weight of instrument (not including UPS; loaded with core consumables)	1296 lb
Crated weight of instrument	1591 lb (722 kg)
Accessories pallet weight	525 lb (238 kg)

Documents

• NovaSeq X Series – In-depth specs sheet (PDF)
• NovaSeq X Series – Product brochure
• How to maximize performance on the NovaSeq X Series (Technical note)

Related Products

• NovaSeq 6000
• NovaSeq X Series Reagent Kits
• Illumina DNA PCR-Free Prep
• Illumina DNA Prep